High-resolution imaging of mitochondrial DNA replication
About us
The University of Wollongong (UOW) is a globally recognised institution that combines world-class research with an exceptional academic environment. Home to the Molecular Horizons Institute, UOW sits at the forefront of scientific discovery, equipped with cutting-edge facilities including advanced cryo-electron microscopy (cryo-EM) platforms and custom-built single-molecule fluorescence microscopes. Our mission is to push the boundaries of molecular life sciences, answering critical biological questions that deepen our understanding of cell function and integrity.
The Molecular Horizons Institute fosters an inclusive and dynamic research culture that values collaboration, innovation, and diversity. With strong support from the university and access to extensive resources, we are committed to training the next generation of researchers in cutting-edge methodologies.
The research group
Led by Dr Jacob Lewis and Dr Lisanne Spenkelink, our laboratory pioneers the study of genome stability and replication fidelity by integrating single-molecule fluorescence and cryo-EM techniques. We focus on fundamental questions about how mitochondrial DNA replication is orchestrated – an essential process for proper cellular function and energy production.
The successful PhD candidate will join a collaborative environment that spans national and international partners. You will have access to Molecular Horizons’ state-of-the-art imaging facilities and robust support networks to further your research and professional development.
The project
Funded by the ARC, this project investigates the architecture, dynamics, and fidelity of the mitochondrial replisome. By applying single-molecule imaging and cryo-EM, we aim to understand how key protein complexes interact with mitochondrial DNA to ensure accurate replication. Through this work, you will contribute to fundamental mechanistic insights into how cells maintain and regulate their mitochondrial genome. This knowledge is crucial for understanding the core principles of cellular energy homeostasis and genome stability.
Key responsibilities
- Undertake independent PhD research aligned with the project’s objectives.
- Design and implement experiments using cryo-EM and single-molecule fluorescence methodologies.
- Collect, analyse, and interpret complex datasets to deepen our understanding of mitochondrial DNA replication.
- Prepare and contribute to high-quality manuscripts for peer-reviewed journals.
- Collaborate with internal and external partners, including other PhD students and postdoctoral researchers.
- Present findings at national and international conferences.
- Maintain thorough documentation of research activities, fostering a culture of rigour and reproducibility.
Why join us?
By joining Molecular Horizons, you will be part of a vibrant community dedicated to pushing the frontiers of molecular research. Our state-of-the-art infrastructure, interdisciplinary research environment, and strong institutional support provide an unparalleled platform for cutting-edge molecular and biophysical studies. This PhD position offers a unique opportunity to gain hands-on experience with advanced imaging technologies while contributing to fundamental breakthroughs in mitochondrial biology.
Faculty: Faculty of Science, Medicine and Health
Study area: Chemistry, Health
Student type: Domestic students, International students
Student status: Current student, Future Students
Scholarship amount
UOW Base Rate ($35,522 p.a.) indexed annually.
Duration
3.5 years
Application process
To apply, submit the following:
- Cover Letter outlining your research interests and suitability for the position.
- Curriculum Vitae, including academic qualifications and any relevant research experience.
Eligibility requirements
The ideal candidate will demonstrate:
Essential:
- Honours or master’s degree in molecular biology, biochemistry, biophysics, or a closely related field.
- Experience in protein purification.
- Strong analytical and organisational skills with a keen eye for detail.
- Ability to work both independently and collaboratively in a multidisciplinary environment.
- Excellent communication skills, both written and oral.
Desirable:
- Experience or interest in working with nucleic acid processing enzymes.
- Exposure to advanced microscopy or structural biology methods.
- Basic coding or data analysis experience (e.g., Python).
Application closing date
15 May 2025
Contact information
Jacob Lewis